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ald [2019-07-10 09:41:36]
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ald [2019-07-10 09:41:36]

L-alanine dehydrogenase
Locus
BSU31930
Isoelectric point
5.12
Molecular weight
39.53 kDa
Protein length
378 aa Sequence Blast
Gene length
1137 bp Sequence Blast
Function
alanine utilization
Product
L-alanine dehydrogenase
Essential
no
E.C.
1.4.1.1
Synonyms
spoVN

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,278,325 3,279,461

Phenotypes of a mutant

The protein

Catalyzed reaction/ biological activity

  • L-alanine H2O NAD = pyruvate NH3 NADH (according to Swiss-Prot)
  • Protein family

  • AlaDH/PNT family (according to Swiss-Prot)
  • Cofactors

  • NADH PubMed
  • Structure

  • 2EEZ (from Thermus thermophilus hb8 mutant, 55% identity, 66% similarity)
  • Localization

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • AdeR: activation, PubMed, in AdeR regulon
  • Regulation

  • induced by alanine (AdeR) PubMed
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    Biological materials

    Mutant

  • BKE31930 (ald::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATCTGTCTCCTCCTGTA, downstream forward: _UP4_TAATTCACAATAAGCTTGCA
  • BKK31930 (ald::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATCTGTCTCCTCCTGTA, downstream forward: _UP4_TAATTCACAATAAGCTTGCA
  • References

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